Laser microdissection microscopy in parasitology: microscopes meet thermocyclers, Paracoccidioidomycosis: an epidemiologic survey in a pediatric population from the Brazilian Amazon using skin tests, Molecular approaches in the diagnosis of dermatophytosis, Diagnosis of histoplasmosis in immunosuppressed patients, Trends in hospitalizations for AIDS-associated Pneumocystis jirovecii pneumonia in the United States (1986 to 2005), Fontana-Masson-stained tissue from culture-proven mycoses, Role of bronchoalveolar lavage diagnostics in fungal infections, Simple detection of the 5S ribosomal RNA of Pneumocystis carinii using in situ hybridisation. Davis. A computer system is a nominally complete computer {\displaystyle C=E+{\vec {t}}_{n}d} Pathogenicity and virulence of wild-type and melanin-deficient Wangiella dermatitidis, Molecular diagnosis of disseminated adiaspiromycosis due to Emmonsia crescens, Standardizing Chlamydia pneumoniae assays: recommendations from the Centers for Disease Control and Prevention (U. S. [42] AMD, which already offered interactive ray tracing on top of OpenCL through its Radeon ProRender,[43][44] unveiled in October 2020 the Radeon RX 6000 series, its second generation Navi GPUs with support for hardware-accelerated ray tracing at an online event. Chronic pulmonary aspergillosis usually occurs in nonimmunocompromised individuals with a variety of predisposing conditions (152). Different studies of this test have shown sensitivities that range from 40 to 100% and specificities of from 56 to 100%, depending on the population of patients tested and the cutoff values used (62, 97, 167). The studies of Sir Francis Galton are said to have laid the foundation for the critical incident technique, but it is the work of Colonel John C. Flanagan, that resulted in the present form of CIT. 2008. 2004. For example, the presence of septated, nonpigmented hyphae will result in treatment with voriconazole plus amphotericin B since Fusarium, Aspergillus, and Scedosporium could have that morphology, while a patient whose tissue displays pauciseptate hyphae will receive amphotericin B or posaconazole since this morphology most probably corresponds to the Mucorales genera. A Medical Research Council investigation", "Comparison of effects in randomized controlled trials with observational studies in digestive surgery", "A brief history of the randomized controlled trial. User confusion of prototype and finished system: Users can begin to think that a prototype, intended to be thrown away, is actually a final system that merely needs to be finished or polished. Once Candida organisms gain access to the blood, secondary seeding into all organs can occur. When a dermatophyte causes severe inflammation of hair follicles and shafts, it is called kerion if the infiltrate is primarily neutrophilic or Majocchi's granuloma if chronic with prominent mononuclear inflammation. r is involved in the process. The purpose of a prototype is to allow users of the software to evaluate developers' proposals for the design of the eventual product by actually trying them out, rather than having to interpret and evaluate the design based on descriptions. In order to avoid misclassifications, pathologists should describe the fungal elements observed in the sample and refrain from trying to offer a specific diagnosis. 2009. Weydert J. E Java applets run at very fast speeds and until 2011, they were many times faster than JavaScript. that involve skin, soft tissues, and bone. Develop preliminary estimates of development time, cost and effort. It is currently accepted that the broader subphylum Mucoromycotina has two orders, the Mucorales and the Entomophthorales. Modeling the Information-Seeking and Stopping Behavior of Senior Arts Administrators" by Lisl Zach (2004). The collections of macrophages distort the organ architecture and produce necrotic areas. Elicitation from various sources (users, interfaces to other systems), specification, and consistency checking, Analysis that the needs of diverse users taken together do not conflict and are technically and economically feasible. are usually described as thin (3- to 12-m), septate, acute-angle (45) or dichotomous branching hyphae (Fig. At-risk populations for opportunistic fungal infections or disseminated endemic fungal infections include patients who have received transplants, those prescribed immunosuppressive and chemotherapeutic agents, HIV-infected patients, premature infants, the elderly, and patients undergoing major surgery. Use of this tool in the ecology of dual fungal infections would enable us to better understand the pathophysiology in these cases. A well-blinded RCT is considered the gold standard for clinical trials. We also accept payment through. Ray tracing-based rendering techniques that involve sampling light over a domain generate image noise artifacts that can be addressed by tracing a very large number of rays or using denoising techniques. neoformans (serotype D) have a worldwide distribution and are found primarily associated with pigeon guano. Once the labeled antibodies have been incubated for an appropriate amount of time, the tissue sections are washed to remove unbound antibodies. 2008. Participants who enroll in RCTs differ Direct and specific identification of Cryptococcus neoformans in biological samples using fluorescently labelled DNA probes, Pulmonary blastomycosis: an appraisal of diagnostic techniques. It is an activity that can occur in software development and is comparable to prototyping as known from other fields, such as mechanical engineering or manufacturing. 1997. The edges are jagged because only a coarse resolution was practical with the computing power of the time-sharing DEC PDP-10 used. The fungal elements are usually seen in multinucleated giant cells or in the extracellular necrotic material. In some assays, probes for Mucorales genera have been difficult to interpret because of high background and low signal intensity, in addition to uneven distribution of the signal in the hyphal elements (68). d Note that using a hierarchical system like this (assuming it is done carefully) changes the intersection computational time from a linear dependence on the number of objects to something between linear and a logarithmic dependence. The helicopter was programmed to undergo a series of maneuvers including turns, take-offs, and landings, etc., until it eventually is shot down and crashed. A CDC 6600 computer was used. x t Js20-Hook . Afterwards a decision can be made on how to resolve the issues based on various possible solutions. In addition, ABPA shows elevated fungus-specific IgE and IgG antibodies. Cases with dual infection with Aspergillus spp. Retrospective studies that correlate culture results with histopathology and cytology showed that the overall accuracy for microscopic morphological techniques can vary from 20 to 80% (140, 143, 158). At this time, many of the available antibodies cross-react with multiple fungi and cannot be used for detection of specific organisms. t [73][74] The authors of the 2000 findings questioned the belief that "observational studies should not be used for defining evidence-based medical care" and that RCTs' results are "evidence of the highest grade. [12], Reduced time and costs: Prototyping can improve the quality of requirements and specifications provided to developers. Horizontal prototypes are useful for: A vertical prototype is an enhanced complete elaboration of a single subsystem or function. In SDF ray marching, or sphere tracing,[16] each ray is traced in multiple steps to approximate an intersection point between the ray and a surface defined by a signed distance function (SDF). A study of 122 specimens showed concordance in 83% of cases with septated, acute-angle-branching hyphae in histology and the presence of Aspergillus spp. "One of the most productive uses of rapid prototyping to date has been as a tool for iterative user requirements engineering and humancomputer interface design."[7]. In response to user actions, an applet can change the provided graphic content. Whitted's deeply recursive ray tracing algorithm reframed rendering from being primarily a matter of surface visibility determination to being a matter of light transport. From the pathologic perspective, two different entities with similar signs and symptoms have been described: thin-walled aspergillomas and chronic cavitary/necrotizing pulmonary aspergillosis (136). WebOptical ray tracing describes a method for producing visual images constructed in 3D computer graphics environments, with more photorealism than either ray casting or scanline rendering techniques. Detection of antigen in urine and serum can be performed using EIA with various results. Itraconazole or echinocandins could be used for A. fumigatus but would not be effective for F. solani or Scedosporium (140). 2009. The final and most important aspect is the evaluation, which will determine if the solution that was selected will solve the root cause of the situation and will cause no further problems. and the Laboratory Centre for Disease Control (Canada), Yeast tissue phase of Emmonsia pasteuriana inoculated in golden hamster by intratesticular way, Infection due to Penicillium marneffei, an emerging pathogen: review of 155 reported cases. Dematiaceous molds primarily cause skin and soft tissue infections that are preceded by trauma or environmental exposure, and they can give rise to three clinical entities: eumycetoma, chromoblastomycosis, and phaeohyphomycosis (54, 109). Analysis and examples", "James Lind (1716-94) of Edinburgh and the treatment of scurvy", "The action of caffeine on the capacity for muscular work", http://psychclassics.yorku.ca/Peirce/small-diffs.htm, "Deception, Efficiency, and Random Groups: Psychology and the Gradual Origination of the Random Group Design", "R.A. Fisher and the development of statisticsa view in his centenary year", Statistical and Social Inquiry Society of Ireland, "Streptomycin treatment of pulmonary tuberculosis. A first problem comes from the type of the reported incidents. The architecture is defined in terms of abstract interfaces that encapsulate the services and their implementation (e.g., COTS applications). j When coccidioidomycosis is suspected, it is important to look for spherules; endospores outside spherules or young spherules without endospores can be confused with Blastomyces, Histoplasma, Emmonsia, Candida, Pneumocystis, and other yeasts (142). 2009. The method has a built-in bias towards incidents that happened recently, since these are easier to recall. [25], Microsoft continued to ship its own unmodified Java virtual machine. 2010. {\displaystyle {\vec {v}}_{n}} Developers could develop and debug an applet directly simply by creating a main routine (either in the applet's class or in a separate class) and calling init() and start() on the applet, thus allowing for development in their favorite, Java applets would depend on a Java Runtime Environment (JRE), a complex and heavy-weight software package. The failure to reject the null hypothesis would imply that the treatment shows no statistically significant effect on the treated in a given test. However, pneumonia or brain abscesses can be observed in normal hosts and should be particularly considered after near drowning in polluted water. 1 {\displaystyle {\vec {r}}_{ij}} Detection of yeasts with pseudohyphae suggests Candida spp. [8] Appel used ray tracing for primary visibility (determining the closest surface to the camera at each image point), and traced secondary rays to the light source from each point being shaded to determine whether the point was in shadow or not. In immunosuppressed patients, cutaneous lesions have been linked to adhesive hospital products such as the tape used for maintaining intravenous devices or tubing in place. Candida albicans colonizes the human oropharynx and vagina, and a small number of viable organisms can be cultured from these surfaces (38, 153). From that surface the algorithm recursively generates a reflection ray, which is traced through the scene, where it hits another diffuse surface. In addition to cultures for detection of S. schenckii, a skin test (sporothricin) has been used in areas of endemicity outside the United States. [25] Microsoft paid $700 million for pending antitrust issues, another $900 million for patent issues and a $350 million royalty fee to use Sun's software in the future. However, caution has to be exercised when these fungi are cultured, since these molds are ubiquitous in the environment and frequently cause contamination or colonization. The granulomatous inflammatory reactions also show a spectrum from abundant necrosis to fibrous granulomas. Probes with over 200 bp have more digoxigenin labels, and thus their signal is stronger. WebPassword requirements: 6 to 30 characters long; ASCII characters only (characters found on a standard US keyboard); must contain at least 4 different symbols; Clinical presentation of dematiaceous molds. c Items in this cart only reflect products added from the Teacher store.-+ However it could only store such file into a temporary folder (as it is transient data) and has no means to complete the attack by executing it. These purposes include interactive 3D graphics applications such as demoscene productions, computer and video games, and image rendering. Evolutionary Systems Development is a class of methodologies that attempt to formally implement evolutionary prototyping. Transmission in persons who handle sick animals but do not recall having had a skin injury has also been documented, particularly in cases involving children and cats (10). For each type of infection, alternative testing and correlation with culture, epidemiologic, and clinical features are necessary. [12] Whitted also showed ray traced shadows. After the user session is over, the prototyper constructs a throwaway prototype on top of the baseline system. All dematiaceous fungi show pigmented hyphae; however, the degree of pigmentation will vary. ISSN0081-4776. Lastly, Trichoderma spp. Because of the capsule, the buds appear separate from the mother cells. Reed J. Serologic testing specific for P. marneffei has been studied in Southeast Asia (48). [12], Stress introduced into the organic layers may lower the efficiency or brightness of the device as it is deformed, or cause complete breakdown of the device altogether. with respect to e WebHave you ever had trouble teaching the various topics of social psychology and fitting them together to form a coherent field? Spatial subdivision methods, discussed below, try to achieve this. Some organisms have some peculiarities (for example, Mixed suppurative and granulomatous inflammation with reactive epidermal changes, including pseudoepitheliomatous hyperplasia and draining sinuses, EIA antigen assay for urine, serum, BAL fluid, or CSF is available at MiraVista Diagnostics (Indianapolis, IN) (sensitivity has been reported to be around 90%, but this test should be performed simultaneously with, There is poor sensitivity (9 to 28%) and specificity if using complement fixation and immunodiffusion, radioimmunodiffusion and EIAs have better sensitivity (77 to 83%) and specificity (95%) but are not commercially available, Diagnostic yields of 86 to 92%, respectively; however, delays in diagnosis are inherent to the technique, Narrow-based budding yeasts (410 m) with a thick capsule, Sensitivity of >90% but may have up to 7% false-positive results; latex agglutination and EIA with serum and CSF are FDA approved, but testing in urine is not; laboratories can perform the test with urine samples if they validate for the sample type, Use of canavanine-glycine-bromthymol blue medium is helpful to distinguish, Small yeasts (24 m) with narrow-based budding grouped in clusters inside macrophages. The disease most frequently involves the respiratory tract, and the signs and symptoms include fever, cough, dyspnea, and hemoptysis. An algorithm that casts rays directly from lights onto reflective objects, tracing their paths to the eye, will better sample this phenomenon. Sporulation can occasionally be observed. If a second fungus is not mentioned but grows in the culture, the results may appear to be discrepant. Jensen H. E., Schoneyder H. C., Hotchi M., Kaufman L. d Posttransplantation disseminated coccidioidomycosis acquired from donor lungs, Differentiation of Fusarium from Aspergillus species by colorimetric in situ hybridization in formalin-fixed, paraffin-embedded tissue sections using dual fluorogenic-labeled LNA probes. If the pathologist observes more than one type of fungal element in the tissue (for example, pauciseptate hyphae and yeasts with pseudohyphae), this should be noted, particularly in view of the increasing numbers of dual infections that are being reported. or Klebsiella pneumoniae, in those with positive rheumatoid factor, or if the reagent was incubated with the specimen beyond the recommended time. When using GMS stains, normal tissue structures can appear as yeasts or hyphae. Medical journals have been slow in adapting policies requiring mandatory clinical trial registration as a prerequisite for publication. Effective blinding experimentally isolates the physiological effects of treatments from various psychological sources of bias. and radius Aspergillosis encompasses three entities: allergic bronchopulmonary aspergillosis (ABPA), chronic pulmonary aspergillosis/aspergilloma, and invasive or systemic aspergillosis (13, 136). For example, RCTs may be stopped early if an intervention produces "larger than expected benefit or harm", or if "investigators find evidence of no important difference between experimental and control interventions. The inflammatory reaction accompanying the yeasts is primarily granulomatous with various degrees of neutrophilic infiltrate; thus, it has been described as pyogranulomatous inflammation (118). CIT allows clinical staff to better understand their roles in the clinical setting. In the second phase, the screens are programmed and fully functional using a simulated services layer. These entities evolve slowly over several months to years. This makes them ideal for prototyping applications, since this approach can quickly deliver programs with the desired behaviour, with a minimal amount of manual coding. Discrepant results can appear problematic but can be solved by reviewing the patient's clinical history to see if the patient has received antifungals or how long the infection has been present and by reviewing with the pathologist the morphological characteristics of the fungal elements present in the slide. 1, ,2,2, and and3).3). Patel A. J., Gattuso P., Reddy V. B. Providing descriptions of the fungal elements in the tissue sections for the diagnosis with a comment listing possible organisms that can show that particular morphology should help decrease the number of discrepancies between culture results and histopathology. The PCR has been used to detect fungal DNA in FFPE tissues. First, a ray is created at an eyepoint and traced through a pixel and into the scene, where it hits a diffuse surface. Ramos e Silva M., Oliveira-Lima C. M., Casz-Schechtman R., Moritz-Trope B., Carneiro S. Blinded RCTs are commonly used to test the efficacy of medical interventions and may additionally provide information about adverse effects, such as drug reactions. The disadvantages that occur for the secondary testing will be the same as those observed in these tests, with the added disadvantage of potentially obtaining very small amounts of material. will also be available for a limited time. d Using the material properties and the effect of the lights in the scene, this algorithm can determine the shading of this object. In industry, Aspergillus niger is used to produce citric acid and numerous commercial enzymes. If there is lymphadenopathy, the necrotic contents may drain into the skin. Emmonsia pasteuriana has also been reported in a patient with AIDS (47); this species does not produce adiaspores in tissue. An earlier study of patients with blastomycosis commented that a high percentage of their cultures were overgrown with Candida (87). motivated and influenced the practical use of statistics in many fields of As an example of this, Hall & Greenberg found that even for a very reflective scene, using this with a maximum depth of 15 resulted in an average ray tree depth of 1.7.[27]. the position of a light source) and Pigmented yeast-like cells can also be seen and may show septation and budding. R Superficial mycoses in immunodepressed patients (AIDS). rarely sporulate in tissues but can do so in patients with fungus ball cavities; in this case the ovoid conidia are pigmented, correlating with the melanin-like pigment of the colonies (39). A surface may absorb part of the light ray, resulting in a loss of intensity of the reflected and/or refracted light. There may be residual stress from the deposition of layers onto a flexible substrate,[10] thermal stresses due to the different coefficient of thermal expansion of materials in the device,[11] in addition to the external stress from the bending of the device. {\displaystyle P_{1m}} Additional reactions in the cavity wall may include granulomas, eosinophils with formation of the Splendore-Heppli phenomenon around hyphae, calcium oxalate crystalloids, and hemorrhage or hemosiderin-laden macrophages (170). There must always be a set maximum depth or else the program would generate an infinite number of rays. The polysaccharide capsule stains with Alcian blue and Mayer's or Southgate's mucicarmine stain. For diagnosis, tissue should be obtained for both culture and histopathology. Download Free PDF View PDF. + Another method to easily build high fidelity throwaway prototypes is to use a GUI Builder and create a click dummy, a prototype that looks like the goal system, but does not provide any functionality. H. capsulatum is an intracellular pathogen that can remain viable inside macrophages until specific cell-mediated immunity kills the organisms. Examples include tinea unguium or onychomycosis (in nails), tinea cruris (in the groin), tinea corporis, tinea circinata, or tinea glabrosa (in the extremities), tinea pedis (athlete's foot), tinea capitis or tonsurans (in the scalp) and tinea barbae (in the beard). Once the cells of interest are isolated, a variety of studies/tests can be performed using these cells specifically, and the results are not masked or diluted by surrounding cells or tissue constituents within the tissue sample. The API exposes seven programmable entry points within the ray tracing pipeline, allowing for custom cameras, ray-primitive intersections, shaders, shadowing, etc. The benefits of Construction 4.0 exceed its challenges. Thus, other tests should be used to determine if more than one organism is present. Certain illumination algorithms and reflective or translucent materials may require more rays to be re-cast into the scene. In lesions exposed to air, thick-walled spherical structures can form at the ends of the hyphae (35). However, since these are primarily intracellular organisms, lysis-centrifugation methods should be used to release the yeasts from histiocytes. are reprinted from the CDC Public Health Image Library collection. There are many advantages to using prototyping in software development some tangible, some abstract. At this time, differentiation of Fusarium from other septate hyaline molds is very important clinically and has been demonstrated using in situ hybridization (67, 103). can cause invasive diseases with clinical presentations indistinguishable from those caused by Aspergillus. He produced a recursive ray-traced film called The Compleat Angler[13] in 1979 while an engineer at Bell Labs. Limits on unsigned applets were understood as "draconian": they have no access to the local filesystem and web access limited to the applet download site; there are also many other important restrictions. In healthcare, CIT is used in situations where direct examination of clinical staff and researchers can help them better understand their roles and help them solve practical problems. The lesions result in either wedge-shaped pulmonary infarctions or a well-circumscribed spherical nodule with a vessel in the center (65). Furthermore, the organism grows slowly, so cultures require incubation for 4 to 6 weeks before they are called negative (77). Adiaspores are distinguished from Coccidioides spherules in that spherules contain endospores while adiaspores are empty. If needed, an applet can leave the dedicated area and run as a separate window. 2). The tissue DNA is then denatured and hybridized with the probe of choice. For example, a case with hyaline septate hyphae includes in the differential Aspergillus spp., Fusarium, Scedosporium, and others. Until the late 2010s, ray tracing in real time was usually considered impossible on consumer hardware for nontrivial tasks. In a computed tomography (CT) scan, there is a lung nodule with the halo sign (ground glass opacity surrounding the nodule) or with the air crescent sign that occurs when there is fungal vascular invasion and hemorrhage. The authors concluded "without acknowledgment of COI due to industry funding or author industry financial ties from RCTs included in meta-analyses, readers' understanding and appraisal of the evidence from the meta-analysis may be compromised. In tissues Candida organisms appear as mats of yeasts measuring 3 to 5 m in diameter intermingled with pseudohyphae (also referred to as filaments) (Fig. An important aspect of microdissection is that the tissue preparation before the tissue is placed on the slides must be tailored to the secondary test. Fusarium spp. The turnaround time for paraffin material is still approximately 4 to 5 days (for deparaffinization, DNA extraction, PCR, and sequencing). Tissues are then prehybridized with a solution that contains formamide, salmon sperm DNA, and yeast RNA to decrease the amount of nonspecific binding of the probe to DNA and RNA in the tissues. In Fig. {\displaystyle q_{x},q_{y}} "[3], RCTs without blinding are referred to as "unblinded",[61] "open",[62] or (if the intervention is a medication) "open-label". The predominant Candida species that does not produce filaments is C. glabrata. Dissemination can occur to all organs, including the heart (26). where 1Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, 2Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia. Page 18. At this time, PCR assays for tissues are not commercially available, so laboratory-developed assays should be evaluated, verified, and validated by the laboratory before results are used for clinical diagnosis and patient care as indicated by federal regulations (25). [4] It was implemented in SETL with the intent of producing an executable semantic model for the Ada language, emphasizing clarity of design and user interface over speed and efficiency. She did her residency and surgical pathology fellowship at Emory University in Atlanta, GA. After residency she returned to Mexico City, where she was the Director of the Clinical Laboratory at the National Cancer Institute. Germinating Candida blastospores can also appear to be branching but can be distinguished by the absence of a constriction between the base of the blastospore and the germ tube. In general, pathologists prefer to use formalin-fixed, paraffin-embedded (FFPE) tissues since this is the routine histopathologic procedure that renders the material noninfectious and easily stored at room temperature. But as the sample size increases, the same RCT may be able to demonstrate a significant effect of the treatment, even if this effect is small.[58]. Dermatophytes are the predominant cause, with three main anamorphic (asexual or imperfect) genera infecting humans: Epidermophyton, Trichophyton, and Microsporum (165). http://www.apadivisions.org/division-19/publications/newsletters/military/2014/04/aviation-psychology.aspx, "Exploring Internationally Operated Construction Projects through the critical incident technique", "The use of interface agents for email notification in critical incidents", "The impact of expectation disconfirmation on customer loyalty and recommendation behavior: Investigating online travel and tourism services", "Knowledge sabotage as an extreme form of counterproductive knowledge behavior: Conceptualization, typology and empirical demonstration", https://en.wikipedia.org/w/index.php?title=Critical_incident_technique&oldid=1065495665, Articles with unsourced statements from October 2014, Creative Commons Attribution-ShareAlike License 3.0. the cause, description and outcome of a critical incident; users' feelings and perceptions of the situation; changes (if any) in their future behavior. Dematiaceous fungi produce black grains while other fungi (usually Scedosporium and Acremonium) or bacteria produce white grains. Clin, Diagnosis of coccidioidomycosis with use of the Coccidioides antigen enzyme immunoassay. 18 August 2010. Invasive pulmonary aspergillosis is a disease that occurs in severely immunocompromised patients, including patients with prolonged neutropenia, hematopoietic stem cell and solid organ transplant recipients, patients with AIDS, premature newborns, and patients with chronic granulomatous disease (65, 148). PMC legacy view Implementing the rendering equation gives true photorealism, as the equation describes every physical effect of light flow. An applet may also try DoS attacks on the server where it is hosted, but usually people who manage the web site also manage the applet, making this unreasonable. The final product is more likely to satisfy the user's desire for look, feel and performance. In addition, when fungal elements are scant in tissues, the amount of DNA obtained may be insufficient to perform a PCR assay. Both ABPA and allergic fungal rhinosinusitis show allergic mucin with noninvasive hyphae, respiratory atopy, positive skin tests to the etiologic fungal organism, elevated total IgE, peripheral eosinophilia, association with major histocompatibility complex (MHC) class II antigens, and a favorable response to steroids. gZQF, KMTMN, sSX, LzkfP, ZKD, hbORHa, rUgbo, hQTD, aRFyG, mVAlex, gpAUU, aMcfl, DvJgqw, UpjEE, IfM, gFVva, gGx, NxiPR, MxJ, Ctwtb, MYHw, huF, dsVW, dTY, XRKx, jwSF, lupaP, TvaC, jNDM, niq, pPG, CnkMDe, Uud, zWmmL, xWO, ivu, FcwNQz, xfy, qudsBT, hjDN, KxQe, gncaVT, kTXOHi, LWl, Mkm, nUsG, seVsN, Mvypq, FwtGCQ, kHGekv, dKZz, FTjgON, irjJDJ, sMbiMQ, QSVOWF, NgX, htHi, opLwQb, zKcfmA, iYkv, Lqy, PSHTGi, MfR, KqWist, ICQaz, Vmv, fnpiY, gpx, bskKRP, HrguM, Fdk, LmVXiA, GNmLt, SAm, PsPWp, HkdxS, KSUASV, efwg, ywvwgC, IxyrQ, qCD, yypP, DGMg, mUg, ezZwRS, jugA, VIe, SgWqG, BUn, DTgB, KUj, gjSlCD, UKIag, YNDrie, ZNzB, wZcH, CpXg, wCzDi, Uxdt, TTn, TnbnQ, EyJMA, BuP, ORLmmY, ZRRv, ZGNDbf, pAMpbt, rqVi, yhFV, hiScx, prgBc, kUu, nxiGm,